t 75 culture flasks greiner (Greiner Bio)
94
Structured Review
Greiner Bio
t 75 culture flasks greiner
T 75 Culture Flasks Greiner, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 94/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+dishes+100/Cell+Culture+Dish+Ps+100%2F20+Mm/pm41920742-47-27-30
Average 94 stars, based on 202 article reviews
T 75 Culture Flasks Greiner, supplied by Greiner Bio, used in various techniques. Bioz Stars score: 94/100, based on 202 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cell+culture+dishes+100/Cell+Culture+Dish+Ps+100%2F20+Mm/pm41920742-47-27-30
Average 94 stars, based on 202 article reviews
t 75 culture flasks greiner - by Bioz Stars,
2026-10
94/100 stars
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other:Article Title: Organoid culture systems for prostate epithelial and cancer tissue Article Snippet: Nicotinamide (Sigma-Aldrich, cat. no. N0636) N-acetyl-l-cysteine (Sigma-Aldrich, cat. no. A9165) A83-01 (Tocris Bioscience, cat. no. 2939) Y-27632 dihydrochloride (Abmole Bioscience, cat. no. M1817) Human fibroblast growth factor (FGF)-10 (PeproTech, cat. no. 100-26) Human FGF-2 (PeproTech, cat. no. 100-18B) Human epidermal growth factor (EGF) (PeproTech, cat. no. AF-100-15) Recombinant human Noggin (Peprotech, cat. no. 120-10C) R-spondin 1–conditioned medium (instructions for preparation are in Box 1), or recombinant R-spondin 1 protein (R&D Systems, cat. no. 4645-RS-025) Prostaglandin E2 (Tocris Bioscience, cat. no. 2296) SB202190 (Sigma-Aldrich, cat. no. S7076) (DiHydro)testosterone (5α-Androstan-17β-ol-3-one) (Sigma-Aldrich, cat. no. A8380) FBS (Sigma-Aldrich, cat. no. F7524) DNase I) from bovine pancreas (Sigma-Aldrich, cat. no. D5025) DAPI (Life Technologies, cat. no. D1306) RosetteSep human CD45 depletion cocktail (StemCell Technologies, cat. no. 15122) Ficoll-Paque PLUS (GE Healthcare Life Sciences, cat. no. 17-1440-02) Recovery cell culture freezing medium (Life Technologies, cat. no. 12648-010) RNeasy mini kit (Qiagen, cat. no. 74104) Reliaprep gDNA tissue miniprep system (Promega, cat. no. A2052) GoScript reverse transcriptase (Promega, cat. no. A5003) Oligo(dT)15 primer (Promega, cat. no. C1101) Recombinant RNasin RNase inhibitor (Promega, cat. no. N2511) Mouse or human material (see Reagent Setup for further details of appropriate sources) EQUIPMENT Falcon tubes, 15 ml Falcon tubes, 50 ml SepMate-50 (StemCell Technologies, cat. no. 15450) Polystyrene round-bottom tube with cell-strainer caps, 5 ml (Falcon) Microcentrifuge tubes, 1.5 ml Shaking platform, 37 °C Plates, 6 well ( Cell Culture:Article Title: Organoid culture systems for prostate epithelial tissue and prostate cancer tissue Article Snippet: CD26-FITC conjugated antibody (anti-human 1:200, M-A261, eBioscience) CD49f-alexa 647 conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd49f-PE conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd24-alexa 647 conjugated antibody (anti-mouse 1:200, 30-F1, eBioscience) .. Falcon tubes 15 ml Falcon tubes 50 ml 5 ml polystyrene round-bottom tube with cell-strainer caps (Falcon) Microcentrifuge tubes, 1.5 ml 37°C shaking platform Plates 6-well (Greiner Bio-One, cat. no. 657 160) Plates 12-well (Greiner Bio-One, cat. no. 665 180) Plates 24-well (Greiner Bio-One, cat. no. 662 160) Plates 48-well (Greiner Bio-One, cat. no. 677 180) Light Microscopy:Article Title: Organoid culture systems for prostate epithelial tissue and prostate cancer tissue Article Snippet: CD26-FITC conjugated antibody (anti-human 1:200, M-A261, eBioscience) CD49f-alexa 647 conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd49f-PE conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd24-alexa 647 conjugated antibody (anti-mouse 1:200, 30-F1, eBioscience) .. Falcon tubes 15 ml Falcon tubes 50 ml 5 ml polystyrene round-bottom tube with cell-strainer caps (Falcon) Microcentrifuge tubes, 1.5 ml 37°C shaking platform Plates 6-well (Greiner Bio-One, cat. no. 657 160) Plates 12-well (Greiner Bio-One, cat. no. 665 180) Plates 24-well (Greiner Bio-One, cat. no. 662 160) Plates 48-well (Greiner Bio-One, cat. no. 677 180) Dissection:Article Title: Organoid culture systems for prostate epithelial tissue and prostate cancer tissue Article Snippet: CD26-FITC conjugated antibody (anti-human 1:200, M-A261, eBioscience) CD49f-alexa 647 conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd49f-PE conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd24-alexa 647 conjugated antibody (anti-mouse 1:200, 30-F1, eBioscience) .. Falcon tubes 15 ml Falcon tubes 50 ml 5 ml polystyrene round-bottom tube with cell-strainer caps (Falcon) Microcentrifuge tubes, 1.5 ml 37°C shaking platform Plates 6-well (Greiner Bio-One, cat. no. 657 160) Plates 12-well (Greiner Bio-One, cat. no. 665 180) Plates 24-well (Greiner Bio-One, cat. no. 662 160) Plates 48-well (Greiner Bio-One, cat. no. 677 180) Microscopy:Article Title: Organoid culture systems for prostate epithelial tissue and prostate cancer tissue Article Snippet: CD26-FITC conjugated antibody (anti-human 1:200, M-A261, eBioscience) CD49f-alexa 647 conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd49f-PE conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd24-alexa 647 conjugated antibody (anti-mouse 1:200, 30-F1, eBioscience) .. Falcon tubes 15 ml Falcon tubes 50 ml 5 ml polystyrene round-bottom tube with cell-strainer caps (Falcon) Microcentrifuge tubes, 1.5 ml 37°C shaking platform Plates 6-well (Greiner Bio-One, cat. no. 657 160) Plates 12-well (Greiner Bio-One, cat. no. 665 180) Plates 24-well (Greiner Bio-One, cat. no. 662 160) Plates 48-well (Greiner Bio-One, cat. no. 677 180) FACS:Article Title: Organoid culture systems for prostate epithelial tissue and prostate cancer tissue Article Snippet: CD26-FITC conjugated antibody (anti-human 1:200, M-A261, eBioscience) CD49f-alexa 647 conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd49f-PE conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd24-alexa 647 conjugated antibody (anti-mouse 1:200, 30-F1, eBioscience) .. Falcon tubes 15 ml Falcon tubes 50 ml 5 ml polystyrene round-bottom tube with cell-strainer caps (Falcon) Microcentrifuge tubes, 1.5 ml 37°C shaking platform Plates 6-well (Greiner Bio-One, cat. no. 657 160) Plates 12-well (Greiner Bio-One, cat. no. 665 180) Plates 24-well (Greiner Bio-One, cat. no. 662 160) Plates 48-well (Greiner Bio-One, cat. no. 677 180) Hood:Article Title: Organoid culture systems for prostate epithelial tissue and prostate cancer tissue Article Snippet: CD26-FITC conjugated antibody (anti-human 1:200, M-A261, eBioscience) CD49f-alexa 647 conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd49f-PE conjugated antibody (anti-human/mouse 1:200, GoH3, BD Biosciences) Cd24-alexa 647 conjugated antibody (anti-mouse 1:200, 30-F1, eBioscience) .. Falcon tubes 15 ml Falcon tubes 50 ml 5 ml polystyrene round-bottom tube with cell-strainer caps (Falcon) Microcentrifuge tubes, 1.5 ml 37°C shaking platform Plates 6-well (Greiner Bio-One, cat. no. 657 160) Plates 12-well (Greiner Bio-One, cat. no. 665 180) Plates 24-well (Greiner Bio-One, cat. no. 662 160) Plates 48-well (Greiner Bio-One, cat. no. 677 180) |